GOLD MEDAL INEPO 2010

GOLD MEDAL INEPO ISTANBUL TURKEY 2010
SEMESTA BILINGUAL BOARDING SCHOOL

UTILIZATION OF SOYA LIQUID WASTE FOR SINGLE CELL PROTEIN PRODUCTION
SHEYLA LESTARINI, FILA ARIFRIANI, COUNSELOR:DWI ELDINA S.PD
Abstract
Soya liquid waste is a big problem for environment especially in the river system. This problem should be solved by reducing, reusing, recycling and increasing the values of soya liquid waste. Soya liquid waste is a benefit product that contains organic compounds which is consisted of carbohydrate, lipid and protein which are useful as production substrates of Single Cell Protein (SCP). This is a new innovation that is needed to be developed. Observation about utilization of soya liquid waste for Single Cell Protein (SCP) production with using Lactobacillus acidophilus bacteria and Bifidobacteria sp has done.
This research was done by producing MRSB and MRSA media, doing sterilization of equipments and materials, doing inoculation of Lactobacillus acidophilus and Bifidobacteria sp aseptically into the Erlenmeyer tube that contains MRSB. The process of growth step was done by using shaker for two weeks. The pureness checking of colony Lactobacillus acidophilus bacteria and Bifidobacteria sp microscopically by doing Gram staining, and then the centrifugation process was done to get pellet. Put the pellet on the petri disc and move it into the oven with temperature of 30oC. The dried pellet from the oven was moved immediately into a mortar. It was mixed with 2 gram of lactose as carrier. The last step from our research was put the pellet result from two separated bacteria with 2 gram lactose into the capsules.
In this research, we produced SCP supplement in the form of capsules. Based on the composition analysis of the product it was obtained that the protein rates of Single Cell Protein (SCP) production from soya liquid waste by using Lactobacillus acidophilus bacteria and Bifidobacteria sp are 35.1 % and 39.9 % of protein. The 5.27 % content of pure soya liquid waste shows very significant increased protein content compared with the 39% and 35.1% of Single Cell Protein (SCP) production from soya liquid waste by using Lactobacillus acidophilus bacteria and Bifidobacteria sp . It is increased reach to 700-800% higher than pure soya liquid waste. This information shows that soya liquid waste can be used as the base of Single Cell Protein (SCP) development of organic waste utilization.